Figure 1.

Baicalin effect on HCEM cell proliferation. (A) Daily changes in the quantification of the HCEM cell number with the addition of baicalin. HCEM cells were seeded at a density of 1.0 × 10⁴ cells in 24-well plates with the addition of 0–1 μM baicalin on the next day. The number of live cells was counted using trypan blue solution at 0, 3, and 5 days after baicalin addition. There was an increase in the HCEM cell number over time by day 5 of culture. However, there was no significant difference between the baicalin-supplemented and non-supplemented groups at 3 and 5 days after plating (Fig. 1A). ∗∗p < 0.01, ∗p < 0.05, n = 3. (B) Analysis of cell proliferation activity using BrdU immunoassay. Compared with the control group, treatment with 0.01, 0.1, or 1 μM baicalin did not significantly alter HCEM cell proliferative activity (Fig. 1B). ∗∗p < 0.01, ∗p < 0.05, n = 16.