Fig. 2.

a Fluorescence (Hoechst nuclei—blue and BODIPY LBs—green, magnification 20×, NA = 0.5) and coherent anti-Stokes Raman scattering images (CARS, magnification 40×, NA = 1.1) for control and stimulated EoL-1 cells. b The relative number of LBs, shown as a graph of the number of LBs per number of cells, calculated from fluorescence images (N of control cells: 1430, BA: 2620, TNF: 870, Il-1β: 840, LPS: 3730). Values are given as mean of the 4 images means ± SEM between the images and are shown in box plots: mean (horizontal line), SEM (box), minimal and maximal values (whiskers). *p < 0.05, **p < 0.01, ***p < 0.001. c Multi-parametric morphological analysis of LBs (upper) and nuclei (lower) from fluorescence images. The significance (*p < 0.05, **p < 0.01, ***p < 0.001) refers only to comparison of control and BA cells and it is shown underneath the parameters in brackets