Table 2.
Run metrics* |
Sample coverage** |
|||||||
---|---|---|---|---|---|---|---|---|
ISP density, % | yield total reads (M) | useable reads | % Polyclonal | mapped reads | % on target | mean depth | uniformity, % | |
FNAB | 94 | 22.6 | 64% | 31 | 1,380,181 | 96 | 2,430 | 93 |
FFPE | 93 | 18.7 | 53% | 38 | 1,326,909 | 96 | 2,288 | 93 |
Acceptable | >70 | 10–30 | >30% | <50 | >200,000 | >80 | ≥500X | ≥80 |
FFPE, formalin-fixed paraffin-embedded; FNAB, fine-needle aspiration biopsy.
Run metrics were averaged from 9 separate runs for FNAB samples and 8 separate runs for FFPE samples.
Sample coverage data were averaged from the 68 FNAB samples and 37 FFPE samples used in the validation study.