Fig. 5.
High osteoclastogenic potential and increased activity of osteoclastic cells derived from female Panx1-deficient mice. A. Panx1 expression in cells isolated from 6-week-old mice at the time of seeding and after 4 day-treatment with M-CSF/RANKL. B Gene expression in bone marrow cells cultured for 4 days under osteoclastogenic conditions. C and D osteoclast number and area of ex vivo osteoclasts cultured for 4 days on plastic. Images are representative of osteoclast cultures. E. CTX-I levels in the media measured/well (total) or corrected by the number of osteoclasts, and relative pit area of ex vivo-generated osteoclasts cultured for 3 days on bone slices. F. Non-adherent bone marrow cells were cultured in the presence of regular media or with phenol red-free (PR-free) α-MEM supplemented with charcoal-stripped fetal bovine serum (CS-FBS) for 3 days. N = 8/group for A-E, 4/group for F. Bars indicate means and lines standard deviations, and each dot corresponds to an individual sample. *: p < 0.05 vs. Panx1fl/fl mice for the same sex.