Fig. 8.

Reduced mineralization in Panx1-deficient male mice. A-B. Ex vivo mineralization assay of bone marrow stromal cells cultured in osteoblastogenic media alone (A), or with conditioned media from osteoclasts of either genotype and the same sex (B). C. Gene expression in stromal osteoblastic cells treated for 14 days with osteoblastogenic media. D. Sost expression in the sixth lumbar vertebra from Panx1^ΔOC mice and Panx1^fl/fl littermate controls. N = 3–12 /group (A-B), 4–8 (C), or 9–12 (D). Bars indicate means and lines standard deviations, and each dot corresponds to an individual sample. *: p < 0.05 vs. Panx1^fl/fl mice for the same sex.