Skip to main content
. 2021 Dec 24;12:791095. doi: 10.3389/fimmu.2021.791095

Figure 2.

Figure 2

IgA+ plasma cells show little evidence of recirculation. (A, B) C57BL/6 (CD45.2) and B6.CD45.1 adult females were connected by parabiosis and were monitored for blood sharing. After 17 days, mice were euthanized and the frequency of donor plasma cells in BM (A) and siLP (B) was determined by flow cytometry using CD45.1/CD45.2. (C, D) B6.Blimp1+/GFP adults were given an intravenous (i.v.) injection of PE-anti-CD19 (C) or PE-anti-CD138 antibodies (D) and sacrificed two minutes later. BM cells were stained with the indicated antibodies and 2 x 106 events were collected to identify cells bound by either PE-labeled antibody in situ. Grey histograms represent the B cells from naïve control mice. (E) Frequency of cells bound by PE-anti-CD138 antibodies in the indicated tissue. Each symbol represents an individual mouse; red lines and error bars indicate the mean and SEM for each group. Gates are shown in (C). Representative of 2 independent experiments with 4 mice per group. * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001.