Figure S3. Knockout of RB1 increases UV susceptibility at the TERT promoter.

(A) Dose dependent inhibition of qPCR amplification of MECOM locus, a highly susceptible locus, in wild-type (WT) cells (García-Nieto et al, 2017). Relative qPCR amplification is compared with GAPDH, a relatively protected locus in WT cells. UV doses are 0, 100, 200 J/m² UVB. (B) qPCR inhibition of the TERT promoter in both RB1 KO and WT cells after 100 J/m² UVC exposure. Primers amplify −224 to −308 of TERT promoter and includes ETS sequences. Three technical replicates were used for each sample. Standard error was calculated using error propagation.