Figure 3. Up-regulated interferon-stimulated gene (ISG) expression in ADAR1-deficient endothelial cells (ECs).
(A) ECs isolated from ADAR1EC-KO mice were analyzed for ISG expression levels. Real-time RT-PCR was used to quantify a panel of 23 ISG expression activities. Significantly increased expression was observed in 16 genes in the cells of EC-KO mice compared with the littermate controls. P < 0.05, n = 3 (control) and 4–6 (EC-KO). (B) Expression of seven selected ISGs was also quantified in cultured ECs isolated from inducible ADAR1 KO (i-KO) mice. ADAR1 gene deletion was induced by adding tamoxifen to the culture medium. Non-induced ECs (NT) and ECs isolated from wild type mice were used as controls. The expression of all these genes was significantly increased in tamoxifen-treated i-KO ECs. P < 0.05, n = 3 (control) and 3–4 (i-KO). RNA expression levels were determined using the ∆∆Ct method with average of GAPDH and HPRT as endogenous control.