Figure 8. MDA-5 deletion rescues ADAR1EC-KO mice from postnatal death.
(A) ADAR1EC-KO mice were crossed with MDA5−/− mice and in the resulting double knockout (d-KO) mice, both ADAR1 and MDA-5 were deleted in ECs. (B) PCR genotyping was used to monitor the genetic status of floxed ADAR1, Cad-5 Cre, and MDA-5 and identify the d-KO mice. Panel (B) shows the typical genotypes of the breeders, progenies of F1 and F2 generations. (C) The postnatal death that occurred to ADAR1EC-KO was not observed in the d-KO mice. The d-KO mice survived to adulthood without noticeable abnormality. (D) ADAR1 gene deletion in the ECs isolated from 6-wk-old d-KO mice was tested using PCR analysis and compared with 2-wk-old ADAR1EC-KO mice. Efficient ADAR1 deletion was observed in d-KO mice.