Skip to main content
. 2021 Dec 30;5(3):e202101191. doi: 10.26508/lsa.202101191

Figure S6. Multiple editing sites were verified in B1 short interspersed nuclear element (SINE) RNA transcripts.

Figure S6.

RNA of wild type endothelial cells was subjected to Sanger sequencing analysis after RT-PCR, which amplifies the selected B1 SINE elements. In the chromatographs of the sequencing, guanosine (G) peaks were seen together with adenosine (A) peaks. (A, B, C) Panel A shows the editing sites in the B1 element in the intron 14 of the Sppl2a gene (chr2: 126892815-126892950), whereas the editing sites in the 3′ UTR of the Nipa1 gene (chr7: 55977578-55977700) and the 3′ UTR of the Slfn5 gene (chr11: 82962556–82962680) are shown in panels (B and C). The sequences of each SINE are shown on the top with the editing sites highlighted. At the bottom are the corresponding sequencing chromatographs; the arrows indicate the editing sites.