Figure S8. Western analysis of ADAR1 protein expressed in endothelial cells (ECs).

Total protein prepared from cultured ECs that were isolated from wild-type (WT) and inducible ADAR1 KO mice (i-KO) was analyzed by Western blot with the anti-ADAR1 monoclonal antibody, clone 15.8.6 (against the RNA-binding domains). Tamoxifen induction on i-KO ECs almost completely removed ADAR1 protein from the cells. No truncated protein was detected.