Figure S8. Relationship between total junction reads and percent spliced in (PSI) difference between biological replicates.

The left panel shows the relationship between total reads count (all junction reads, log₂ transformed) and PSI difference between replicates based on the original dataset. The right panel shows the relationship between original read count (log₂ transformed) and PSI difference estimated based on the down-sampled dataset. The red line in each scatter plot was fitted by linear regression. Pearson’s correlation coefficient (R) and P-value are presented for each subfigure.