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. 2021 Aug 26;17(1):451–458. doi: 10.1016/j.jds.2021.07.026

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© 2021 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Candida albicans and fungi levels quantification in saliva and occlusal plaque. Fungi-specific (ITS1, ITS2) and the C. albicans-specific (SAP) primers were used to evaluate the levels of fungi and C. albicans using qPCR. Percentage of total fungi (A), and C. albicans (B) within the total fungi, in the saliva and occlusal plaque collected from subjects with (FOA,) and without fixed orthodontic appliances (Control,). The groups significant differences were analyzed with one-way ANOVA followed by Kruskal–Wallis multiple comparisons test; ∗P <0.01, ∗∗P < 0.001, ∗∗∗P < 0.0001.

Candida albicans and fungi levels quantification in saliva and occlusal plaque. Fungi-specific (ITS1, ITS2) and the C. albicans-specific (SAP) primers were used to evaluate the levels of fungi and C. albicans using qPCR. Percentage of total fungi (A), and C. albicans (B) within the total fungi, in the saliva and occlusal plaque collected from subjects with (FOA,) and without fixed orthodontic appliances (Control,). The groups significant differences were analyzed with one-way ANOVA followed by Kruskal–Wallis multiple comparisons test; ∗P <0.01, ∗∗P < 0.001, ∗∗∗P < 0.0001.