Figure 5.

Exocyst trafficks MVBs by interacting with Rab11a

(A) Exocyst interacts with Rab11a, as assessed using the STRING database. (B–E) Co-immunoprecipitation (co-IP) assay results showed that Sec10, Sec3, Exo70, and Rab11a interacted in HN4 and CNE2 cells. (F and G) Representative images of immunohistochemical staining (brown) (F) and summary data showing Rab11a expression in LC tissue versus adjacent healthy tissue (Ctrl) (G). ∗p < 0.05 versus siCtrl by t test. Scale bar, 50 μm (H and I) Exosome concentrations after the cells were transfected with scrambled or Rab11a siRNA in HN4 cells. (H) Representative nanoparticle tracking analysis traces. (I) The quantification of exosome concentrations in HN4 cells. ∗∗p < 0.01 versus siCtrl by t test. (J) Representative western blotting images show that CD9, CD63, and CD81 each interact with Rab11a, Exo70, Sec3, and Sec10 in HN4 and CNE2 cells. Lys, whole-cell lysates; Pre-immu, preimmune serum. (K–Z) The interactions between CD63 and Exo70, Sec3, Sec10, or Rab11a after siRNA-mediated of Exo70, Sec3, Sec10, or Rab11a knockdown. CD63 was used for normalization. ∗p < 0.05, ∗∗p < 0.01 versus siCtrl by t test.