TABLE 3.

The end-point, relative fluorescence (ΔRn), results of PCR tests for 100 rough and problematic Salmonella isolates^a

Test no.	Strain no.	Results		Test no.	Strain no.	Results
		Sample (FAM)b	Control (TET)c			Sample (FAM)b	Control (TET)c
1	9820995-19	3.0	2.7	51	9823052	2.2	2.3
2	9821012-26	2.9	2.8	52	9823078-12	2.1	2.4
3	9821020-6	3.2	3.0	53	9822835-2	2.4	2.7
4	9821004-13	3.0	2.7	54	9822922-2	2.1	2.3
5	9820925-6	2.6	2.7	55	9823078-18	2.1	2.2
6	9820928-2	3.3	3.1	56	9822922-4	2.4	2.4
7	9820925-1	2.5	2.8	57	9823078-21	2.1	2.4
8	9820997-10	3.0	2.9	58	9823071-1	2.1	2.3
9	9820965-4	2.9	2.7	59	9823000	2.1	2.3
10	9820995-2	1.7	2.2	60	9823054-3	2.0	2.3
11	9821087-9	2.8	2.4	61	9823050-1	2.4	2.5
12	9821087-13	2.2	2.4	62	9822985-1	2.3	2.6
13	9821070	2.9	2.7	63	9823078-13	2.2	2.3
14	9821067-2	2.5	2.3	64	9823047-10	2.3	2.5
15	9821067-5	2.1	2.4	65	9823078-16	2.2	2.4
16	9821068-2	2.3	2.6	66	9823078-23	2.2	2.3
17	9820913-14	2.3	2.4	67	9822990	2.0	2.2
18	9821087-11	2.3	2.3	68	9822869	1.9	2.1
19	9820964-4	2.5	2.5	69	9823078-22	2.8	2.6
20	9821062-7	2.5	2.6	70	9822757-10	2.8	2.2
21	9821012-21	3.3	3.1	71	9823071-5	3.6	2.7
22	9820965-5	2.5	2.8	72	9822999	2.7	2.2
23	9825150-1	2.7	2.5	73	9822991	3.0	2.2
24	9825120-1	2.5	2.3	74	9823071-7	3.5	2.8
25	9824500-1	2.8	2.8	75	9823078-11	2.8	2.5
26	9820997-3	2.4	3.0	76	9823097-10	1.3	1.5
27	9824687-1	3.3	2.7	77	9823078-6	2.9	2.2
28	9825120-1	2.4	2.4	78	9823097-4	4.0	3.1
29	9824332-1	2.5	2.4	79	9823071-6	3.2	2.7
30	9824973-4	3.6	2.9	80	9822823	2.3	2.3
31	9824640-1	2.2	2.6	81	9823092-1	2.9	2.2
32	9825075-1	2.6	2.5	82	9823078-10	3.6	2.8
33	9824966-1	2.5	2.5	83	9822922-1	2.9	2.7
34	9820995-1	2.7	2.6	84	9823078-17	2.6	2.4
35	9820995-2	2.5	2.8	85	9823071-8	4.0	3.1
36	9820925-6	2.4	2.7	86	9823074	3.1	2.9
37	9820995-19	3.6	3.4	87	9823073-2	2.7	2.7
38	9920144-1	2.7	2.8	88	9823071-9	2.9	2.3
39	9820995-18	3.0	3.3	89	9823023-2	3.4	2.5
40	9820997-10	2.5	2.7	90	9823097-9	4.1	2.9
41	9820997-12	2.4	2.6	91	9823078-15	2.6	2.4
42	9820928-2	2.9	2.8	92	9823078-14	2.7	2.3
43	9820925-1	2.3	2.6	93	9823078-3	2.8	2.4
44	9822987-4	2.1	2.4	94	9823078-5	3.7	3.0
45	9823071-4	2.1	2.3	95	9823028-4	2.8	2.6
46	9823071-2	2.0	2.3	96	9823029-1	2.9	2.3
47	9822835-13	1.7	2.3	97	9822864-1	1.1	1.4
48	9822835-14	1.0	1.9	98	9822864-2	1.3	1.9
49	9822835-21	2.1	2.0	99	9822970	1.4	2.0
50	9823019	1.2	1.7	100	9922656-10	1.8	1.2

^aStrains with an uncertain reaction in conventional biotyping or serotyping schemes. 

^bFAM layer values of ≤0.5 were considered negative, values of 0.5 to 0.6 were retested, and values of >0.6 were considered positive. 

^cTET layer values of ≤0.4 were considered negative, values of 0.4 to 0.5 were retested, and values of >0.5 were considered positive.