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. 2021 Dec 24;9:775642. doi: 10.3389/fcell.2021.775642

FIGURE 5.

FIGURE 5

Impacts of the HIF-1α/HECTD2 axis on RCC cell growth in vivo. HIF-1α overexpression and/or HECTD2 knockdown cell model was used for constructing xenografted tumor model in nude mice. The mice were sacrificed at the 35th day after cell transplantation. (A–C): Tumor volume and weight. (D): IHC was adopted to count the number of Ki67-positive cells in 786-O and A-498. (E): The profiles of HIF-1α and HECTD2 in 786-O and HECTD2 cells were monitored by WB. (F,G): The fluorescence intensity of HIF-1α and HECTD2 was checked by tissue immunofluorescence. ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. N = 5. Scale bar = 50 μm.