Table 1.

Comparison of our study and previous studies regarding gut microbiota in mice that have undergone endurance exercise.

Study	Ours	Allen et al., 2015	Lamoureux et al., 2017 *	Allen et al., 2015	Campbell et al., 2016	Evans et al., 2014	Choi et al., 2013
n	12	10	11	10	9	5–6	6
Substrain	C57BL/6 N	C57BL/6 J	C57BL/6 N	C57BL/6 J	C57BL/6 N	C57BL/6 J	C57BL/6 N
Age (weeks)	8	6	6–10	6	6	5	44
Caged	6–8	Individually	Individually	Individually	Individually	Individually	Individually
Diet (energy) Protein Fat Carbohydrates	3.1 kcal/g 24% 18% 58%	3.0 kcal/g 29% 17% 54%	3.20 kcal/g 26% 14% 60%	3.0 kcal/g 29% 17% 54%	3.82 kcal/g 20% 10% 70%	3.85 kcal/g 20% 10% 70%	n.a.
Exercise	FTR	FTR	FTR	VWR	VWR	VWR	VWR
Intervention time (weeks)	4 (5 days/week)	6 (5 days/week)	6 (5 days/week)	6 (5 days/week)	12 (7 days/week)	12 (7 days/week)	5 (7 days/week)
Distance/session	1,000 m	480 m	600-700-800 m	5,836 ± 132 m/night	17,390 ± 6,890 (Counts)	11714.28 m	11538.06 m
Time/session	60 min	40 min	40 min	n.a.	n.a.	n.a.	680 min
Speed	12–24 m/min	8–12 m/min	15–20 m/min	n.a.	n.a.	n.a.	18.67 m/min
Exercise intensity	High	Low	Moderate	n.a.	n.a.	n.a.
Group training	Yes	No	No	No	No	No	No
Sample origin	cecal	cecal	fecal	cecal	fecal	fecal	fecal
16S rRNA sequencing	V2-4-8, V3-6, and V7-9 sequencing	V3 and V5 sequencing	V6-V8 sequencing	V3 and V5 sequencing	TRFLP and pyrosequencing	TRF and V4 sequencing	PhyloChip Arrays
Relevant taxa (vs. Sedentary mice)	↓Ruminoccocus gnavus ↑Parabacteroides ↑Desulfovibrio	↑R. gnavus ↑Clostridium ↑Butyrivibrio ↑Oscillospira ↑Coprococcus	↓Ruminoccocus ↓Lactobacillus ↓Clostridiales ↓Parabacteroides ↑Bacteroides ↑Lachnospiraceae	↑Coprococcus ↑Turicibacter	↑Allobaculum spp. ↑Ruminoccocus ↑Clostridiales ↑Akkermansia	Exercise increased the Bacteroidetes:Firmicutes ratio in a manner that was proportional to the distance run.	↓Ruminoccocus

n.a.: Data not available.

^*This study includes both male (n = 5) and female (n = 6) mice, the rest of the studies were conducted on males.