TABLE 1.
Characteristics of MAbs used to determine antigens on H. pyloria
| Specificity | Clone | Isotype | Ig concn (μg/ml) | Dilutions used in ELISA and inhibition ELISA | Reference |
|---|---|---|---|---|---|
| HpaA | 30:1:1:6 | IgG1 | 65 | 1/100 | 5 |
| Flagellins (FlaA+FlaB) | 48:13 | IgG1 | 40 | 1/100 | 24 |
| Urease 30-kDa subunit | 8:1 | IgG1 | 42 | 1/100 | I. Bölin and A.-M. Svennerholm, unpublished data |
| 26-kDa protein | 18:1 | IgG1 | 34 | 1/100 | 24 |
| NAP | 6:8 | IgG1 | 31 | 1/100 | D. G. Evans and A.-M. Svennerholm, unpublished data |
| Lea (type 1 chain) | T174 | IgG1 | 175 | 1/2,000 | 29, 35 |
| Leb (type 1 chain) | T218 | IgM | 810 | 1/2,000 | 29, 35 |
| Lex (type 2 chain) | P12 | IgM | 175 | 1/2,000 | 29, 35 |
| Sialyl-Lex | CSLEX1 | IgM | 50 | 1/2,000 | 29, 35 |
| Ley (type 2 chain) | F3 | IgM | 125 | 1/2,000 | 29, 35 |
a
Anti-Lewis MAbs were purchased from Signet Laboratories, Inc.