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. 2021 Dec 24;12:809541. doi: 10.3389/fphar.2021.809541

FIGURE 3.

FIGURE 3

The treatment with fluoxetine or vortioxetine is needed to rescue the expression of Gpx1 at gene but not at protein level. Effects induced by i.c.v. administration of Aβ oligomers (Aβ + VEH) in absence or presence of FLX10, VTX5, or VTX10 on (A) Gpx1 mRNA expression measured by qRT-PCR; (C) monomer and (E) dimer of Gpx1 protein levels measured by WB. (B, D) show the representative immunoblots of Gpx1 monomer (22 kDa) and Gpx1 dimer (44 kDa) in total protein extracts from hippocampal tissue. In the case of gene expression measurements, the abundance of Gpx1 mRNA was expressed relative to the abundance of GAPDH-mRNA, as an internal control. As a negative control, a reaction in absence of cDNA (no template control, NTC) was performed. qRT-PCR amplifications were performed at least in triplicate (mean of three to five determinations). In the case of protein expression measurements, histograms refer to the means ± S.E.M. of the densitometric values of Gpx1 monomer or Gpx1 dimer bands normalized against β-actin (42 kDa). Each experiment was repeated three times. *p < 0.05 vs. PBS + VEH, # p < 0.05 vs. Aβ oligomers + VEH, ### p < 0.001 vs. Aβ oligomers + VEH.