TABLE 1.
Protein identification from Naja samarensis venom fractions isolated by reverse-phase high-performance liquid chromatography using nano-ESI-LCMS/MS.
| RP-HPLC derived fractions/Protein name | Database accession | Species annotated | Protein score | Relative abundance (%) |
| Fraction 1 | 65.87 | |||
| Short neurotoxin 1 | P60774 | N. samarensis | 96.24 | 27.93 |
| Short neurotoxin 1 | P01424 | N. melanoleuca | 42.31 | 34.87 |
| Cobrotoxin | P60770 | N. atra | 22.22 | 3.07 |
| Fraction 2 | 3.86 | |||
| Muscarinic toxin-like protein 2 | P82463 | N. kaouthia | 46.73 | 3.86 |
| Fraction 3 | 4.46 | |||
| Weak toxin CM-2 | P01415 | N. haje | 17.79 | 4.46 |
| Fraction 4 | 3.98 | |||
| Acidic phospholipase A2 natratoxin | A4FS04 | N. atra | 68.39 | 1.93 |
| Acidic phospholipase A2 D | Q9I900 | N. sputatrix | 57.64 | 1.83 |
| Cytotoxin 1 | P01467 | N. mossambica | 40.65 | 0.11 |
| Cytotoxin 3 | P01470 | N. mossambica | 37.27 | 0.11 |
| Fraction 5 | 6.93 | |||
| Cytotoxin 2 | P01441 | N. oxiana | 162.93 | 1.86 |
| Cytotoxin 10 | P86541 | N. naja | 78.78 | 0.65 |
| Cytotoxin 2 | P01445 | N. kaouthia | 74.29 | 0.37 |
| Cytotoxin SP15c | P60308 | N. atra | 73.38 | 3.26 |
| Cytotoxin homolog | P14541 | N. kaouthia | 59.57 | 0.15 |
| Cytotoxin 1 | P01467 | N. mossambica | 50.54 | 0.27 |
| Venom nerve growth factor 2 | Q5YF89 | N. sputatrix | 30.51 | 0.13 |
| Hemextin A | P0DQH3 | H. haemachatus | 18.59 | 0.23 |
| Cysteine-rich venom protein ophanin | Q7ZT98 | O. hannah | 16.61 | 0.01 |
| Fraction 6 | 9.36 | |||
| Cytotoxin 2 | P01441 | N. oxiana | 108.69 | 1.00 |
| Cytotoxin 2a | P86538 | N. naja | 62.12 | 1.74 |
| Cytotoxin 1 | P01447 | N. naja | 52.31 | 5.87 |
| Cytotoxin 1 | P01467 | N. mossambica | 30.39 | 0.70 |
| Zinc metalloproteinase-disintegrin-like atrase-B | D6PXE8 | N. atra | 24.83 | 0.07 |
| Fraction 7 | 5.54 | |||
| L-amino-acid oxidase | P81383 | O. hannah | 111.89 | 0.26 |
| Natrin | *CL2736.Contig1_NsM2 | N. atra | 100.47 | 0.42 |
| Cysteine-rich venom protein natrin-1 | Q7T1K6 | N. atra | 78.40 | 0.33 |
| Cysteine-rich venom protein ophanin | Q7ZT98 | O. hannah | 65.76 | 0.16 |
| Cysteine-rich venom protein ophanin-like | XP_026568636.1 | P. textilis | 48.83 | 0.14 |
| Zinc metalloproteinase-disintegrin-like atrase-A | D5LMJ3 | N. atra | 79.58 | 0.21 |
| Metalloproteinase atrase A | *CL115.Contig7_NkT | N. atra | 41.01 | 0.20 |
| Metalloproteinase | B5G6F7 | D. vestigiata | 49.02 | 0.15 |
| Zinc metalloproteinase-disintegrin-like MTP4 | F8RKW1 | D. coronoides | 19.50 | 1.22 |
| Zinc metalloproteinase-disintegrin-like atrase-B | D6PXE8 | N. atra | 22.21 | 2.22 |
| Hemorrhagic metalloproteinase-disintegrin-like kaouthiagin | P82942 | N. kaouthia | 22.92 | 0.10 |
| Ohanin | P83234 | O. hannah | 20.66 | 0.13 |
Relative abundance (%) refers to the abundance of individual proteins in a fraction, which was estimated based on the mean spectral intensity (MSI) of the peptide ions and the peak area under curve of its corresponding chromatographic fractions. Bold values are subtotals of protein abundances per fraction.
*Protein codes with suffix “_NsM2” and “_NkT” indicate venom proteins identified based on tryptic peptides matched to sequence from an in-house transcript database containing RNAseq, specific for the following: NsM: Naja sumatrana, Malaysia and NkT: Naja kaouthia, Thailand. Mass spectrometric data and peptide sequences are available in Supplemental file.
Abbreviations: D. coronoides, Drysdalia coronoides; D. vestigiata, Demansia vestigiata; H. haemachatus, Hemachatus haemachatus; N. atra, Naja atra; N. haje, Naja haje; N. kaouthia, Naja kaouthia; N. melanoleuca, Naja melanoleuca; N. mossambica, Naja mossambica; N. naja, Naja naja; N. oxiana, Naja oxiana; N. samarensis, Naja samarensis; N. sputatrix, Naja sputatrix; NCBI, National Centre for Biotechnology Information; O. hannah, Ophiophagus hannah; P. textilis, Pseudonaja textilis; RP-HPLC, reverse-phase high performance liquid chromatography.