TABLE 2.
Relative abundance of different toxin families identified from Naja samarensis venom.
| Protein family/Name | Fraction(s) | Database accession | Species | Relative abundance (%) |
| Three-finger toxin (3FTx) | 90.48 | |||
| Short-chain alpha-neurotoxin (SαNTX) | 65.87 | |||
| Short neurotoxin 1 | 1 | P01424 | N. melanoleuca | 34.87 |
| Cobrotoxin | 1 | P60770 | N. atra | 3.07 |
| Short neurotoxin 1 | 1 | P60774 | N. samarensis | 27.93 |
| Cytotoxin (CTX) | 16.29 | |||
| Cytotoxin 1 | 6 | P01467 | N. mossambica | 0.70 |
| Cytotoxin 2 | 5,6 | P01441 | N. oxiana | 2.84 |
| Cytotoxin 2 | 5 | P01445 | N. kaouthia | 0.37 |
| Cytotoxin 1 | 6 | P01447 | N. naja | 5.87 |
| Cytotoxin 1 | 4,5 | P01467 | N. mossambica | 0.38 |
| Cytotoxin 3 | 4 | P01470 | N. mossambica | 0.11 |
| Hemextin A | 5 | P0DQH3 | H. haemachatus | 0.23 |
| Cytotoxin homolog | 5 | P14541 | N. kaouthia | 0.15 |
| Cytotoxin SP15c | 5 | P60308 | N. atra | 3.26 |
| Cytotoxin 2a | 6 | P86538 | N. naja | 1.74 |
| Cytotoxin 10 | 5 | P86541 | N. naja | 0.65 |
| Muscarinic toxin-like protein (MTLP) | 3.86 | |||
| Muscarinic toxin-like protein 2 | 2 | P82463 | N. kaouthia | 3.86 |
| Weak toxin (WTX) | 4.46 | |||
| Weak toxin CM-2 | 3 | P01415 | N. haje | 4.46 |
| Snake venom metalloproteinase (SVMP) | 4.17 | |||
| Metalloproteinase | 7 | B5G6F7 | D. vestigiata | 0.15 |
| Zinc metalloproteinase-disintegrin-like atrase-A | 7 | D5LMJ3 | N. atra | 0.21 |
| Zinc metalloproteinase-disintegrin-like atrase-B | 6,7 | D6PXE8 | N. atra | 2.29 |
| Zinc metalloproteinase-disintegrin-like MTP4 | 7 | F8RKW1 | D. coronoides | 1.22 |
| Hemorrhagic metalloproteinase-disintegrin-like kaouthiagin | 7 | P82942 | N. kaouthia | 0.10 |
| Metalloproteinase atrase A | 7 | *CL115.Contig7_NkT | N. atra | 0.20 |
| Phospholipase A2 (PLA2) | 3.76 | |||
| Acidic phospholipase A2 natratoxin | 4 | A4FS04 | N. atra | 1.93 |
| Acidic phospholipase A2 D | 4 | Q9I900 | N. sputatrix | 1.83 |
| Cysteine-rich secretory protein (CRiSP) | 1.06 | |||
| Cysteine-rich venom protein natrin-1 | 7 | Q7T1K6 | N. atra | 0.33 |
| Cysteine-rich venom protein ophanin | 5,7 | Q7ZT98 | O. hannah | 0.17 |
| Cysteine-rich venom protein ophanin-like | 7 | XP_026568636.1 | P. textilis | 0.14 |
| Natrin | 7 | *CL2736.Contig1_NsM2 | N. atra | 0.42 |
| L-amino-acid oxidase (LAAO) | 0.26 | |||
| L-amino-acid oxidase | 7 | P81383 | O. hannah | 0.26 |
| Venom nerve growth factor (vNGF) | 0.13 | |||
| Venom nerve growth factor 2 | 5 | Q5YF89 | N. sputatrix | 0.13 |
| Vespryn (VES) | 0.13 | |||
| Ohanin | 7 | P83234 | O. hannah | 0.13 |
The percentages (%) refers to the relative abundances of proteins identified from the HPLC-derived venom fractions. Bold values are subtotals of protein abundances per toxin family or subfamily.
*Protein accession numbers with suffixes “_NsM2” and “_NkT” indicate proteins matched to sequences from an in-house transcript database containing RNAseq, of the following cobras: NsM: Naja sumatrana, Malaysia and NkT: Naja kaouthia, Thailand. Mass spectrometric data and peptide sequences are available in Supplemental File.
Abbreviations: D. vestigiata, Demansia vestigiata; D. coronoides, Drysdalia coronoides; H. haemachatus, Hemachatus haemachatus; N. atra, Naja atra; N. haje, Naja haje; N. kaouthia, Naja kaouthia; N. melanoleuca, Naja melanoleuca; N. mossambica, Naja mossambica; N. naja, Naja naja; N. oxiana, Naja oxiana; N. samarensis, Naja samarensis; N. sputatrix, Naja sputatrix; O. hannah, Ophiophagus hannah; P. textilis, Pseudonaja textilis.