FIGURE 3.

Ablation of neurons using NTIRE. (A) Coronal section highlighting the location of microelectrodes on the cortex. (B) A magnified view of the highlighted region in (A). Significant cell loss is seen down to a depth of approximately 400 μm. Control sections (left panel) highlight the density of cells without the NTIRE ablation. (C) Finite-element method simulations representing the penetration of the electric field into the tissue during one pulse of the NTIRE ablation (detailed discussion of NTIRE pulses in Supplementary Figure 1). The depth of the electric field perpendicular to the microelectrodes. Clearly, the depth of cell loss at approximately 400 μm corresponds to an electric field of 800 V/cm. Values of electric field below this number seem to not cause NTIRE ablation. In the inset, absorption of the electric field is shown in the cortex.