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. 2021 Dec 24;12:752211. doi: 10.3389/fphar.2021.752211

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Copyright © 2021 Bian, Zhang, Tang, Fei, Hu, Chen, Su, Fei, Ji, Mao, Tong, Yuan and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of ZSS extract on body weight (A), pentobarbital sodium-induced sleep latency (B) and sleep duration (C), the levels of 5-HT (D) and GABA (E). Histopathological observation of the hypothalamus at ×200 magnification (F). Con, control group; PCPA, PCPA-induced insomnia model group; Est, estazolam-positive group; ZSS, alcohol extract of Ziziphi Spinosae Semen group. ^** p < 0.01 compared with the control group, ^## p < 0.01 compared with the model group. Data shows mean ± SD values and n = 6.