Fig. 5.

210M transfected astrocytes exhibit an anti-inflammatory and neuroprotective phenotype. Primary human astrocytes were transfected with 210S or 210M, with or without prior exposure to hypoxic and inflammatory stress (“H-I”) using 1% oxygen and interleukin 1-beta, respectively. A, C RT-qPCR assessment of CXCL10, IL6, IGF1 gene expression. B, D CXCL10, IL6, and IGF1 protein levels in cell supernatant measured by ELISA. E, F RT-qPCR assessment of C3 and Sema5b gene expression. All data are presented as mean ± SEM of n = 9 (A, C and E), 5 (B and D) or 7 (F) donors. Statistical comparisons between groups were made by a one-way ANOVA (A and E) or by a paired t-test (B–D, F). *p < 0.05; **p < 0.01