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. 2022 Jan 6;20:24. doi: 10.1186/s12951-021-01232-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — a Representative imaging of lateral side of lymphatic vessels in the tail, ear, flank and lower leg after intradermal injection of P20D680. b Specificity of tracer uptake by lymphatic vessels and popliteal vein after intradermal injection into hind paw with IRDye 800CW (ca.2 nm) and P10D800 (ca.7 nm). c The schematic diagram of lymphatic vessels network in mouse hind limbs (i) and the visualization of high uptake of P20D680 after intradermal injection into hind paw (ii). (iii) The valve function of collecting lymphatic vessels was also visualized in real time by P40D800. d Cartoon of lymphatic flow and rerouting of lymphatic flow after tumor spread from footpad tumors. Reproduced with permission from [124]. Copyright 2013, Elsevier