Fig. 2.

Primary cilia in the embryonic and postnatal cerebral cortex. Primary cilia (green) in the ventricular zone of the developing mouse cerebral cortex (E16) were labeled with hGFAP-Cre induced Sstr3-GFP (A). Radial glia and intermediate progenitors were co-labeled with RC2 (red) and anti-Tbr2 (blue) antibodies, respectively. (B) Live imaging of a primary cilium in the embryonic cerebral wall (E16), over 5.5 h, indicates its dynamic nature. Images at different time points were pseudocolored to illustrate the changing shape, length, and orientation. (C) Projection neuronal primary cilia in postnatal brains (P7) were labeled with Nex-Cre induced Sstr3-GFP (B). Neurons across cortical layers display primary cilia of varying shapes and orientation (C).