Figure 2.

Comparisons of pimonidazole labeling (A–B) and Western blot analysis of α-SMA expression (C–D) in scleras of eyes with transected temporal ciliary arteries or sham operations, one week after surgery. (A) First row, hypoxia signals (green). Second row, hypoxia signals (green) plus DAPI nuclear staining (blue). Third row, higher magnification details of the boxed scleral areas in the second row. Fellow, eyes contralateral to those with either transected temporal ciliary arteries or sham operations; Treated, eyes subjected to either transection of the temporal ciliary arteries or sham surgery. *P < 0.05, **P < 0.01, and ***P < 0.001; independent t-test, Mann-Whitney U test, or Wilcoxon signed rank test, as appropriate.