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Absolute quantification of Filifactor alocis in healthy, chronic and aggressive subjects of periodontitis. The 16srDNA cloned plasmid was used as a standard to quantify Filifactor alocis in the subjects (a). The average absolute count of the bacteria in the various subjects were estimated by pooling of paper points of individual subjects and absolute count quantified in triplicates using quantitative polymerase chain reaction (b)
