FIGURE 4.

EFAMIX deconvolution of experimental SEC‐SAXS data from BSA (a) and glucose isomerase (b); calculations were performed within a two‐component approximation. Column 1—Elution profiles of SEC‐SAXS data obtained by chromatography inline X‐ray scattering (CHROMIXS) (green). Column 2—Restored concentration profiles of the components, the blue and red curves are individual components, and the green curve is the overall concentration profile. Column 3—Restored scattering profiles of the components (blue and red curves, respectively); the fits from the crystallographic models (brown; BSA: 4F5S.pdb, Glucose isomerase: 1OAD.pdb) to the restored scattering profiles by EFAMIX from the most significant component (blue). Column 4—Plots of the forward EFA (solid lines) and the backward EFA (circles) for which the notations and color schemes are the same as in Figure 1. BSA, Bovine serum albumin; EFA, evolving factor analysis; SEC‐SAXS, size‐exclusion chromatography–small‐angle X‐ray scattering