Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 6;139(1):73–86. doi: 10.1182/blood.2021012327

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by The American Society of Hematology

This article is made available via the PMC Open Access Subset for unrestricted reuse and analyses in any form or by any means with acknowledgment of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

PMC Copyright notice

Figure 5. — SOX9 modulates cholesterol biosynthesis via DHCR24. (A) Total and free cholesterol levels were measured in DLBCL cells with high (Karpas-422 and DB) or low (OCI-LY1 and SUDHL8) levels of SOX9. A total of 1 × 10⁶ cells were used for each measurement. (B-D) Total and free cholesterol levels were measured in SOX9⁻ Karpas-422 cells and DB cells and in SOX9-overexpressing OCI-LY1 cells. The level of cholesterol ester was calculated: total cholesterol − free cholesterol content. (E) Immunoblot assay and densitometry quantitation of DHCR24 protein in SOX9⁻ Karpas-422 and DB cells. (F) Karpas-422 or DB cells were treated with triparanol for 48 hours or left untreated before they were subjected to cholesterol content measurement. (A-D, F) Data represent the mean ± SD of technical triplicates. *P < .05; **P < .01; ***P < .001.