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. 2022 Jan 7;18(1):e1010232. doi: 10.1371/journal.ppat.1010232

Fig 1. SPAG9 is upregulated in vIRF1-transduced HUVECs and KSHV-infected HUVECs.

Fig 1

(A). Western-blotting analysis of SPAG9 in HUVECs transduced with lentiviral-vIRF1 or its control lentiviral-pHAGE (MOI of 2). (B). Western-blotting analysis of SPAG9 in HUVECs treated with PBS (PBS) or infected by KSHV wild-type virus (KSHV) (MOI of 3). (C). Western-blotting analysis of SPAG9 expression in HUVECs treated with PBS (PBS) or infected with wild-type KSHV (KSHV_WT) (MOI of 3) or vIRF1 mutant virus (K9_mut) (MOI of 3) for 30 h. (D). Hematoxylin and eosin (H&E) staining and immunohistochemical staining (IHC) of KSHV LANA, SPAG9 in normal skin, skin KS of patient #1 (Skin KS1), patient #2 (Skin KS2), and patient #3 (Skin KS3). Magnification, ×200, ×400. (E). Results were quantified in (D). Data were shown as mean ± SD. *** P < 0.001, Student’s t-test.