Fig 10. Expression of the uppS-cdsA operon.
(A) Cultures of BW25113; ΔyhcB; ΔyhcB/PuppS and ΔyhcB/PcdsA strains grown in LB, normalised to an OD600 of 1.00 and 10-fold serially diluted before inoculating LB agar plates supplemented with vancomycin or SDS and EDTA. (B) RT-PCR data presented as the relative fold change in abundance of uppS and cdsA transcripts in a yhcB mutant compared to the BW25113 control. Data are normalised to gyrA prior to comparative analyses. Data were collected for three biological replicates. The experiment was repeated twice, a representative plot is shown. (C) Cultures of BW25113; BW25113/PcdsAAS; ΔyhcB and ΔyhcB/PcdsAAS strains grown in LB for 5 h, normalised to an OD600 of 0.05 and 10-fold serially diluted before inoculating LB agar plates supplemented with vancomycin or SDS and EDTA. The PcdsAAS construct contains the antisense nucleotide sequence of cdsA. Expression was not induced, leaky expression was sufficient for complementation purposes.