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Comparison of RT-RPA and RT-qPCR for PRRSV-2 detection
| Ct value | RT-RPA result (P/N) | Coincidence rate (%) |
|---|---|---|
| < 30 | 111/0 | 100 |
| 30–32 | 12/0 | 100 |
| 32–34 | 5/20 | 20 |
| 34–35 | 0/9 | 0 |
| 35-Ct | 0/22 | 100 |
A total of 135 clinical samples and 44 viral isolates from cell culture were used to compare the performance of RT-RPA and RT-qPCR
P positive, N negative
