AnkR is enriched in cerebellar Purkinje neurons, granule cells, and the CN. Immunostaining for AnkR in P56 wild-type, C57BL/6, sagittal mouse (A) hindbrain, scale bar: 500 µm; (B) cerebellar cortex, scale bar: 25 µm; and (C) CN, scale bar: 50 µm. D, Immunostaining for AnkR in one-month Ank1 conditional knock-out mice in cerebellum (top) and cortex (bottom), genotypes indicated. Scale bars: 25 µm. E, Immunostaining for AnkR in three-month (top) and six-month (bottom) Ank1F/F;Pcp2-Cre. Scale bar: 25 µm. ML, molecular layer; PCL, Purkinje neuron layer; GCL, granule cell layer.