Figure 9.

Three-dimensional structures of IgG1 Fc region bound to soluble FcγRIIIa (PDB 5vu0) (left) and the crystal structure of an intact IgG1 molecule (PDB 1hzh).⁵⁹ The location of the FcγRIIIa binding interaction is shown as a blue oval and mapped onto the intact IgG structure. Modifications on residues in EU numbering that were found to be critical in our study of mAb1 IgG1 antibody are also shown as colored spheres on the structures indicating their location relative to FcγRIIIa. Deamidation (N325) is shown in magenta, unknown modification (~P227) is shown in black, and glycosylation (N297) is shown in green