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. 2021 Dec 31;2021:6265553. doi: 10.1155/2021/6265553

Table 1.

Antibodies used to detect HIF-1α and β-actin protein levels and primary and secondary antibodies used in WB.

Antibody Dilution Host species Source
HIF-1α 1 : 300 Mouse Cell Signaling Technology (D5F3M)
β-Actin 1 : 1000 Goat Abcam (ab8229)
Anti-mouse IgG-HRP 1 : 2000 Goat Santa Cruz (sc2005)
Anti-goat IgG-HRP 1 : 5000 Donkey Santa Cruz (sc2020)