Table 1.
Antibodies used to detect HIF-1α and β-actin protein levels and primary and secondary antibodies used in WB.
Antibody | Dilution | Host species | Source |
---|---|---|---|
HIF-1α | 1 : 300 | Mouse | Cell Signaling Technology (D5F3M) |
β-Actin | 1 : 1000 | Goat | Abcam (ab8229) |
Anti-mouse IgG-HRP | 1 : 2000 | Goat | Santa Cruz (sc2005) |
Anti-goat IgG-HRP | 1 : 5000 | Donkey | Santa Cruz (sc2020) |