Figure 1.

Ad-SF7-Fc expresses SF7-Fc and activates NK cells and DCs

(A) HEK-293-C7 cells (n = 3) were control treated with PBS (naive) or infected at an MOI of 1 × 10³ with Ad-null or Ad-SF7-Fc overnight and SLAMF7 gene expression was assessed. BALB/c mice were injected I.V. with 1 × 10¹⁰ v.p. of Ad-null (n = 6), Ad-SF7-Fc (n = 6), or not injected (naive, n = 3), spleens were harvested 10 h later and immune cell phenotype was analyzed by flow cytometry. (B) IFNγ production by NK cells. (C) CD69 expression on NK cells. (D) CD86 expression on cDC cells. (E) CCR7 expression on cDC cells. (F) CD86 expression on macrophages. All data presented as mean ± standard error of the mean and representative of a single experiment. Groups compared with one-way ANOVA with Tukey's multiple comparison test. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; n.s. = not significant.