Figure 3.

NR slightly increases muscle mitochondrial respiratory function in HF-fed control but not CrAT-deficient mice

(A–C) Maximal, ADP-supported mitochondrial oxygen consumption (JO₂) was assayed in isolated skeletal muscle mitochondria fueled by (A) octanoyl-carnitine/malate/ADP/cytochrome c, (B) palmitoyl-carnitine/malate/ADP, or (C) glutamate/malate/ADP/succinate.

(D) Pyruvate titration conducted in the presence of ADP and malate.

(E) Correlation matrix. Red lines indicate the line of best fit through the data points and the blue shaded region represents 95% confidence intervals. Data are represented as mean ± SEM.

(A–E) N = 5–8 per group. (A–D) Data were analyzed by two-tailed Student's t-test. ∗ represents a significant difference between HF- and HF + NR-fed mice. ∗P≤0.05. N represents biological replicates.

See also Figure S5.