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. 2021 Nov 29;101(3):101626. doi: 10.1016/j.psj.2021.101626

Figure 1.

Figure 1

Identification of pET-32a-PLpro recombinant plasmid and purification for IBV PLpro. (A) M1: 1kb DNA Marker; 1: Single restriction digestion identification of recombinant plasmid by SacI; 2: Double restriction digestion identification of recombinant plasmid by SacI and XhoI; 3: IBV-PLpro positive amplification products; M2: BM2000+ DNA Marker. (B) Purification for expression proteins of recombinant plasmid pET-32a-PLpro. M: Protein Marker; 1: Whole bacteria; 2: Bacterial breakage precipitation (inclusion bodies); 3: Purified IBV PLpro. Abbreviations: IBV, infectious bronchitis virus; PLpro, papain-like protease.