Figure 5.

The transcript levels of NF-κB pathway proteins in CEK cells. The CEK cells were infected with 0.1 MOI of IBV and 100 μM myricetin was added to treat for 48 h. CEK cells were collected at 4, 8, 12, 18, 24, 36, and 48 h postinfection, and the total RNA of the cells was extracted and reverse transcribed into cDNA, and the relative expression of each target gene was detected by qRT-PCR. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Abbreviations: CEK, chicken embryo kidney; IBV, infectious bronchitis virus; NF- κB, nuclear factor kappa-B.