Fig. 2.

Microglia are dispensable for core sleep and circadian features (A), Minutes spent in each behavioral state measured by EEG. Measurement of 24 h periods were split into 10 s epochs for classification then summed for the graphic. Left; baseline, right; the 24 h following 6 h of SD. n = 12-16 mice. Data expressed as mean ± SD. Statistical analysis performed using an unpaired two-tailed student's t-test. (B), Relative Delta Power (1–4 Hz Frequency range) normalized to average Delta Power at ZT9-12, the lowest point in a 24 h period. Figure covers 2+ days of continuous recording in a 12:12 light/dark setting. The first 24 h are baseline measurements, followed by 6 h of SD (ZT 24-30) and 24 h of recovery period. Only the preceding 24 h before SD were used for this calculation, to allow for the use of 54 h of continuous recording data. See methods for details on calculation and selection of x-axis data points. Data expressed as mean ± SD n = 12-16 mice. Statistical analysis was performed using two way ANOVA between the two conditions across the entire 54 h period, with post-hoc Bonferroni multiple comparisons tests with corrections at each equivalent time point. Two-way ANOVA found a statistically main effect significant difference (p = 0.0015) between the two time-courses, but no differences after Tukey's test with multiple testing corrections at any individual ZT bin. Related Fig. S3A illustrates the entire power-frequency spectrum. Fig. S3D displays wake time for each condition by hour. (C), Period length measured by wheel running in 24 h dark and 12:12 light:dark conditions. An automated system tracked number of wheel revolutions, and period length was defined as the time between the onset of wheel running activity of subsequent circadian days. n = 8-10 mice per condition, 3 weeks of data averaged for each mouse. Data expressed as mean ± SD. Statistical analysis performed using unpaired two-tailed student's t-test. Related Figs. S3B and C display additional features of circadian periodicity.