TABLE 1.
Detection of NLVs in stools by ELISAs using hyperimmune antisera to rSEV and rCV
| Sample | Result of antigen detection ELISA forb:
|
Result of RT-PCR and Southern hybridization | |
|---|---|---|---|
| SeV | CV | ||
| rSEVa | 1.05 | 0.00 | NTc |
| rCVa | 0.00 | 0.93 | NT |
| 121/89 | 1.31 | 0.01 | + |
| 122/89 | 1.61 | 0.00 | + |
| 124/89 | 0.53 | 0.00 | + |
| 125/89 | 0.23 | 0.00 | − |
| 131/89 | 1.48 | 0.01 | + |
| 1381/93 | 0.35 | 0.04 | + |
| 1382/93 | 1.55 | 0.04 | + |
| 663/99 | 0.03 | 0.62 | + |
| 665/99 | 0.04 | 1.32 | + |
| 666/99 | 0.07 | 2.01 | + |
| 669/99 | 0.04 | 0.51 | + |
| 675/99 | 0.01 | 0.28 | + |
| 676/99 | 0.07 | 1.72 | + |
| 680/99 | 0.05 | 1.62 | + |
| 687/99 | 0.06 | 1.04 | + |
Purified VLPs (2 ng/ml) were used as antigens.
OD values at 492 nm. Homologous titers are shown in boldface.
NT, not tested.