KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Escherichia coli BL21 (DE3)	New England Biolabs	Cat#C2527H
Chemicals, Peptides, and Recombinant Proteins
MBX3132	Sjuts et al., 2016	N/A
Deposited Data
Subnanometer in situ structure of AcrAB-TolC bound with inhibitor	This study	EMD-24609
Cryo-EM structure of AcrAB-TolC bound with inhibitor	Wang et al., 2017	PDB: 5NG5
Cryo-EM structure of AcrAB-TolC bound with antibiotics	Wang et al., 2017	PDB: 5O66
MexAB-OprM efflux pump reconstituted in nanodiscs	Glavier et al., 2020	PDB: 6TA6
Recombinant DNA
pAcBH	Fujihira et al., 2002	N/A
pRSF-TolC	Shi et al., 2019	N/A
Software and Algorithms
EMAN2	Chen et al., 2019	http://eman2.org
UCSF Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
FlexEM	Topf et al., 2008	http://topf-group.ismb.lon.ac.uk/flex-em/
Phenix	Adams et al., 2010	http://www.phenix-online.org/
Coot	Emsley et al., 2010	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
MolProbity	Chen et al., 2009	http://molprobity.biochem.duke.edu/