KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological Samples
New Zealand white rabbit	BioIVT	Cat#RAB00MSCUZN
Chemicals, Peptides, and Recombinant Proteins
Phosphatidylcholine	Avanti	Cat#441601G
1,2-dioleoyl-sn-glycero-3-phosphocholine	Avanti	Cat#850375C
Phosphatidylethanolamine	Avanti	Cat#850725P
Critical Commercial Assays
HiTrap Q HP	Cytiva	Cat#17115401
Deposited Data
Proteoliposomal RyR1 primed model	This paper	PDB ID: 7M6A
Proteoliposomal RyR1 primed map	This paper	EMDB ID: 23692
Proteoliposomal RyR1 open model	This paper	PDB ID: 7M6L
Proteoliposomal RyR1 popen map	This paper	EMDB ID: 23699
Cryo-EM structure of recombinant rabbit Ryanodine Receptor type 1 mutant R164C in complex with FKBP12.6	Iyer et al., 2020	PDB ID: 6WOT
Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 3&4)	des Georges et al., 2016	PDB ID: 5TAQ
Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1&2)	des Georges et al., 2016	PDB ID: 5TAL
Software and Algorithms
cryoSPARC	Punjani et al., 2017	https://cryosparc.com/
Relion	Scheres, 2012	https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page
Leginon	Suloway et al., 2005	https://sbgrid.org/software/titles/leginon
Phenix	Adams et al., 2004	https://www.phenix-online.org/
Coot	Emsley and Cowtan, 2004	https://www.ccpem.ac.uk/
HOLE	Smart et al., 1993	http://www.holeprogram.org/
Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
ChimeraX	Pettersen et al., 2021	https://www.cgl.ucsf.edu/chimerax/
Other
UltrAuFoil Holey gold grids	Quantifoil	R 0.6/1.0, Au 300