| Antibodies |
| goat anti-H/K ATPase α Subunit (ATP4A) |
Santa Cruz Biotechnology |
#sc-167155 |
| rabbit anti-H/K ATPase β Subunit (D-18) (ATP4B) |
Santa Cruz Biotechnology |
#sc-84304 |
| mouse anti-αSmooth Muscle Actin (αSMA) |
Sigma-Aldrich |
#A2547 |
| rabbit anti-αSmooth Muscle Actin (αSMA) |
GeneTex |
#981303701 |
| rabbit anti-βcatenin (β-CAT) |
Santa Cruz Biotechnology |
#sc-7199 |
| rabbit anti-βIII-tubulin (TUJ1) |
Abcam |
#ab18207 |
| rabbit anti-Cadherin 17 (CDH17) |
Sigma-Aldrich |
#HPA023616 |
| mouse anti-Cadherin 17 (CDH17) |
R&D Systems |
#MAB1032 |
| rabbit anti-Calbindin (CLBN1) |
Gift from Kenny Campbell |
N/A |
| mouse anti-CDX2 |
BioGenex |
MU392A-UC |
| rabbit anti-CDX2 |
Cell Marque |
#EPR2764Y |
| mouse anti-Chromogranin A (CHGA) |
DSHB |
#CPTC-CHGA-1 |
| rabbit anti-Claudin 18 (CLDN18) |
Sigma-Aldrich |
HPA018446 |
| goat anti-E-Cadherin (ECAD) |
R&D Systems |
#AF648 |
| rat anti-E-Cadherin (ECAD) |
R&D Systems |
#MAB7481 |
| mouse anti-E-Cadherin (ECAD) |
BD Transduction Laboratories |
#610182 |
| goat anti-FOXF1 |
R&D Systems |
AF4798 |
| rabbit anti-gastric intrinsic factor (GIF) |
Sigma-Aldrich |
HPA040774 |
| mouse anti-Gastric Mucin (MUC6) |
Abcam |
#ab49462 |
| rabbit anti-Gastrin (GAST) |
Dako |
#A0568 |
| goat anti-GATA4 |
Santa Cruz Biotechnology |
#sc-1237 |
| chicken anti-GFP |
Aves Labs |
GFP-1010 |
| goat anti-Ghrelin (GHRL) |
Santa Cruz Biotechnology |
#sc-10368 |
| rabbit anti-Glucagon-like peptide-1 receptor (GLP-1R) |
Novus Biologicals |
#NLS1205 |
| rabbit anti-Histamine |
Immunostar |
#22939 |
| rat anti-Keratin 8 (KRT8) |
Developmental Studies Hybridoma Bank |
#TROMA1 |
| rabbit anti-mucin 2 (MUC2) |
Abcam |
#ab134119 |
| mouse anti-Mucin-5AC (MUC5AC) |
Abcam |
#ab3649 |
| rabbit anti-Nitric oxide synthase (nNOS) |
Abcam |
#ab76067 |
| goat anti-PDX1 |
Abcam |
#ab47383 |
| goat anti-Peripherin |
Santa Cruz Biotechnology |
#sc-7604 |
| mouse anti-Pepsinogen I (PGA3) |
Abcam |
#ab31466 |
| sheep anti-Pepsinogen II (PGC) |
Abcam |
#ab31464 |
| rabbit anti-S100β |
Dako |
#Z031129 |
| rabbit anti-Serotonin (5-HT) |
Immunostar |
#20080 |
| goat anti-Somatostatin |
Santa Cruz Biotechnology |
#sc-7819 |
| goat anti-SOX2 |
Santa Cruz Biotechnology |
#sc-17320 |
| goat anti-SOX2 |
R&D Systems |
AF2018 |
| rabbit anti-SOX2 |
Abcam |
#ab97959 |
| guinea pig anti-Synaptophysin |
Synaptic Systems |
#101004 |
| chicken anti-Tyrosine Hydroxylase (TH) |
Aves Labs |
#TYH |
| Bacterial and virus strains |
| N/A |
N/A |
N/A |
| Biological samples |
| Human Stomach and Duodenum Samples |
Wells Lab CCHMC |
GI-1B-9952 A B |
| Human Antral and Fundic Samples |
Helmrath Lab CCHMC |
14-241, 14-242, 14-243, 14-244 |
| Human Stomach and Duodenum Samples |
CCHMC Pathology Core |
N/A |
| Chemicals, peptides, and recombinant proteins |
| 1× DMEM |
Invitrogen |
Cat #11965-084 |
| 50× B27 supplement |
Thermo Fisher Scientific |
Cat #17504044 |
| 50× B27 supplement w/o Vitamin A |
Thermo Fisher Scientific |
Cat #12587-010 |
| A8301 |
Torcis |
Cat #A83-01 |
| Activin A |
Cell Guidance Systems |
Cat #GFH6 |
| Accutase |
Thermo Fisher Scientific |
Cat #A11105-01 |
| Advanced DMEM:F12 |
Thermo Fisher Scientfic |
Cat #12634-010 |
| Atropine sulfate salt monohydrate |
Sigma-Aldrich |
Cat #A0257 |
| Bethanechol |
Sigma-Aldrich |
Cat # 1071009 |
| C59 |
Cellagen Technology |
Cat #C7641-2s |
| CHIR99021 |
ReproCell |
Cat #04-0004-10 |
| Collagen Type IV |
Thermo Fisher Scientific |
Cat #17104019 |
| Defined fetal bovine serum (dFBS) |
Hyclone |
Cat #SH30070.02 |
| Dispase |
Thermo Fisher Scientific |
Cat #17105-041 |
| DMEM/F12 |
Thermo Fisher Scientific |
Cat #11320033 |
| DMEM/F-12, GlutaMAX |
Thermo Fisher Scientific |
Cat #10565018 |
| EGF |
Sigma-Aldrich |
Cat #1257-0.1MG |
| F12 Media |
Invitrogen |
Cat #11765-054 |
| Fetal Bovine Serum (FBS) |
HyClone |
Cat #SH30071.03 |
| Fibronectin |
Corning |
Cat #354008 |
| Geltrex |
Thermo Fisher Scientific |
Cat #A1413201 |
| HEPES Buffer |
Thermo Fisher Scientific |
Cat #15630080 |
| hESC-qualified Matrigel |
BD Biosciences |
Cat #354277 |
| Insulin from bovine pancreas |
Sigma-Aldrich |
Cat #I4011-50 |
| L-glutamine (100×) |
Thermo Fisher Scientific |
Cat #25030-081 |
| L-NAME |
Sigma-Aldrich |
Cat #N5751 |
| Matrigel Matrix Basement Membrane |
BD Biosciences |
Cat #354234 |
| mTeSR1 media |
Stem Cell Technologies |
Cat #5850 |
| N2 Supplement |
Thermo Fisher Scientific |
Cat #17502-048 |
| Non-essential Amino Acids (100×) |
Thermo Fisher Scientific |
Cat #11140050 |
| Normal donkey serum |
Jackson Immunoresearch Labs |
Cat #017-000-121 |
| Neurobasal Medium |
Thermo Fisher Scientific |
Cat #21103049 |
| PD0325901 |
Stem Cell Technologies |
Cat #72184 |
| Pen/Strep (100×) |
Thermo Fisher Scientific |
Cat #15140-122 |
| PIK90 |
EMD Millipore |
Cat #528117 |
| PMA |
Torcis |
Cat #1201 |
| recombinant human BMP4 |
R&D Systems |
Cat #314-BP |
| recombinant human EGF |
R&D Systems |
Cat #236-EGF |
| recombinant human FGF2 |
R&D Systems |
Cat #233-FB |
| recombinant human FGF4 |
R&D Systems |
Cat #235-F4 |
| recombinant human FGF10 |
R&D Systems |
Cat #345-FG |
| recombinant human Noggin |
R&D Systems |
Cat #6057-NG |
| recombinant human WNT3A |
R&D Systems |
Cat #5036-WN |
| RNAlater
|
Thermo Fisher Scientific |
Cat #AM7020 |
| Scopolamine |
Tocris |
Cat #1414 |
| Thiazovivin |
Tocris |
Cat #3845 |
| TTX |
Tocris |
Cat #1078 |
| Y-27632 dihydrochloride |
Tocris |
Cat #1254 |
| Critical commercial assays |
| Quantitect SYBR Green |
Qiagen |
Cat #204145 |
| Nucleospin RNA |
Macherey-Nagel |
Cat #740955 |
| SuperScript VILO cDNA Synthesis Kit |
Thermo Fisher Scientific |
Cat #11754250 |
| Deposited data |
| N/A |
N/A |
N/A |
| Experimental models: cell lines |
| Human: H1 ES cells; >28 hAGO, >18 NCC, and >10 SM differentiations |
CCHMC Pluripotent Stem cell core / WiCell Research Institute |
NIH hESC-10-0043 |
| Human: H9 ES cells |
CCHMC Pluripotent Stem cell core / WiCell Research Institute |
NIH hESC-10-0043 |
| Human: H9-GAPDH-GFP ES cells, >5 NCC differentiations |
CCHMC Pluripotent Stem cell core |
N/A |
| Human: H9 RFP ES cells, >4 SM differentiations |
CCHMC Pluripotent Stem cell core |
N/A |
| Human: H9 mCherry ES cells, >1 NCC differentiation and >3 SM differentiations |
CCHMC Pluripotent Stem cell core |
N/A |
| Human: iPSC77.3 GFP iPS cells, >6 NCC differentiations and >2 SM differentiations |
CCHMC Pluripotent Stem cell core |
N/A |
| Human: WTC11 AAVS1-CAG-GCaMP6f iPS cells, 2 NCC differentiations |
Bruce Conklin’s Lab at UCSF |
N/A |
| Experimental models: organisms/strains |
| Mouse: NSG (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ) |
Comprehensive Mouse and Cancer Core Facility CCHMC |
N/A |
| Oligonucleotides |
| Please see Table S3. |
| Recombinant DNA |
| N/A |
N/A |
N/A |
| Software and algorithms |
| IMARIS |
Bitplane |
N/A |
| NIS Elements |
Nikon |
N/A |
| LabChart software |
ADInstruments |
N/A |
| Other |
| 8-well micro-slide |
Ibidi |
N/A |
| Organ bath chamber |
Radnoti |
N/A |
| Isometric force transducers |
ADInstruments |
N/A |
