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. 2022 Jan 7;12:53. doi: 10.1038/s41598-021-03990-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ZIKVac structure showing the position of CD4 (green) and CD8 epitopes (blue) envelope protein E Domain III (EDIII) and adjuvant (yellow pentagon) (A). The protein sequence of and ZIKVac sequence without conjugated adjuvants showing the EDIII (red), CD4 epitopes (green), CD8 epitopes (blue) and linkers (black) (B). DNTAN linkers were used to separate EDIII and adjuvant from the epitopes, GPGPG linkers were used between CD4 epitopes and AAY linkers between CD8 epitopes.