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. 2022 Jan 7;12:53. doi: 10.1038/s41598-021-03990-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Discontinuous epitopes present in the vaccine construct. Two epitopes are located at the Envelope Domain III portion of the protein (A and C) with some of the highest scores (0.988 and 0.741) and with 4 and 74 amino acids, respectively. The other discontinuous epitopes are distributed among the CD4 and CD8 epitopes (B, D–J). In yellow are the predicted discontinuous epitopes, in red the domain III from the ZIKV Envelope protein, in green CD4 epitopes, in blue CD8 epitopes and in white the linkers.