Table 1.
Zika virus CD8 peptide selection based on multiparameters using immunoinformatics tools.
| Protein | Epitope0 | Size (aa) | Position | Nº of alleles1 | % of total alleles2 | Immunogenicity score (IEDB)3 | Nº of HLAs w/ Percentile Rank ≤ 204 | Nº of HLAs with SMM IC50 ≤ 500 nM5 | Nº of HLAs with ANN IC50 ≤ 500 nM6 |
|---|---|---|---|---|---|---|---|---|---|
| Capsid Protein | VSPFGGLKRLP (C_24-34) | 11 | 24–34 | 17 | 42.5 | 0.0036 | 12 | 1 | 1 |
| prM Protein | IFRNPGFALAAAAIA (prM_129-143) | 15 | 129–143 | 16 | 34.04 | 0.3976 | 49 | 4 | 8 |
| Envelope Protein E | SQEGAVHTALA (E_260-270) | 11 | 260–270 | 10 | 25 | 0.23948 | 15 | 3 | 3 |
| Envelope Protein E | VRGAKRMAVLG (E_415-425) | 11 | 415–425 | 10 | 25 | − 0.21504 | 15 | 2 | 1 |
| Envelope Protein E | MQTLTPVGRLI (E_349-359) | 11 | 349–359 | 9 | 22.5 | 0.13774 | 26 | 3 | 2 |
| NS1 | WRSVEGELNAILEENGVQL (NS1_68-86) | 19 | 68–86 | 11 | 23.40 | 0.5434 | 34 | 3 | 3 |
| NS2A | ALIAAFKVR (NS2A_78-86) | 9 | 78–86 | 5 | 10.64 | 0.07299 | 8 | 1 | 1 |
| NS2B | SEVLTAVGLICAL (NS2B_5-17) | 13 | 5–17 | 22 | 46.81 | 0.24318 | 40 | 8 | 7 |
| NS3 | VREAIKTRLRTVIL (NS3_209-222) | 14 | 209–222 | 14 | 29.79 | 0.26841 | 34 | 6 | 6 |
| NS4A | GKMGFGMVTLG (NS4A_79-89) | 11 | 79–89 | 9 | 22.5 | 0.01824 | 24 | 4 | 4 |
| NS4A | GAAFGVMEALGT (NS4A_1-12) | 12 | 1–12 | 12 | 30 | 0.17747 | 29 | 4 | 3 |
| NS4B | TPLTLIVAI (NS4B_105-113) | 9 | 105–113 | 13 | 27.66 | 0.20764 | 10 | 2 | 1 |
| NS5 | YMWLGARFLEFEAL (NS5_477-490) | 14 | 477–490 | 17 | 36.17 | 0.6005 | 54 | 6 | 6 |
0—Underlined are the first aminoacids of a 9-mer peptide predicted as having percentile rank ≤ 20 as calculated by IEDB (http://tools.iedb.org/mhci/).
1—Total number of HLA-I covered by the peptide as predicted by Propred1. All 47 alleles were selected, and the percentage of the highest scoring peptides, the Immuneproteasome and the Proteasome filter threshold was set up as 3%.
2—Percentage of HLA-I molecules identified as binders in relation to the total number of HLA-I available.
3—Immunogenicity score obtained with http://tools.iedb.org/immunogenicity/. Positive values are considered immunogenic.
4—Number of HLA that presented percentile rank lower than 20 as predicted by IEDB (http://tools.iedb.org/mhci/).
5—Number of HLAs with SMM IC50 ≤ 500 nM as predicted by IEDB (http://tools.iedb.org/mhci/).
6—Number of HLAs with ANN IC50 ≤ 500 nM as predicted by IEDB (http://tools.iedb.org/mhci/).