Table 2.

Zika virus CD4 peptide selection based on multiparameters using immunoinformatics tools.

Protein	Epitope	Size (aa)	Position	Nº of alleles1	% of total alleles2	Immunogenicity (Percentile Rank)3	Nº of HLAs w/Percentile Rank < 204	Nº of HLAs w/ SMM align IC50 < 500 nM5	Nº of HLAs w/NN align IC50 < 500nM6	IFNepitope Score (SVM)7
Capsid Protein	HGPIRMVLAILAFLR (C_41-55)	15	41–55	37	72.54	24.3268	21	17	14	0.4521
prM Protein	TTSTWVVYGTCHHKK (prM_71-85)	15	71–85	31	60.8	65.45964	4	0	2	− 0.1269
Envelope Protein E	EYRIMLSVHGSQHSG (E_136-150)	15	136–150	33	64.7	40.45516	13	9	12	− 0.1841
Envelope Protein E	DKLRLKGVSYSLCTA (E_296-310)	15	296–310	34	66.6	46.33096	12	2	12	− 0.3609
NS1	SYFVRAAKTNNSFVV (NS1_121-135)	15	121–135	30	58.8	31.18672	11	12	12	0.3363
NS2A	PFVMALGLTAVRLVD (NS2A_196-210)	15	196–210	29	56.8	42.90484	17	11	14	0.2505
NS2B	FAAGAWYVYVKTGKR (NS2B_116-130)	15	116–130	11	27.50	42.51056	14	5	9	0.5012
NS3	LRGLPVRYMTTAVNV (NS3_236-250)	15	236–250	35	68.6	43.8254	12	8	13	0.4513
NS4A	LGIFFVLMRNKGIGK (NS4A_66-80)	15	66–80	40	78.4	25.47468	21	10	14	0.0979
NS4B	HYMYLIPGLQAAAAR (NS4B_119-133)	15	119–133	18	35.29	42.61168	19	10	14	0.0579
NS4B	VAVSSAILSRTAWGW (NS4B_178-192)	15	178–192	38	74.5	49.2798	13	6	6	0.3106
NS5	LLYFHRRDLRLMANA (NS5_766-780)	15	766–780	36	70.6	38.39304	20	8	12	0.2457

1—Total number of HLA-II covered by the peptide as predicted by Propred MHC-II Binding Peptide Predicition Server. All 51 alleles were selected. The threshold was set up as 1%.

2—Percentage of HLA-II molecules identified as binders in relation to the total number of HLA-II available.

3—Immunogenicity percentile rank obtained with http://tools.iedb.org/CD4episcore/. Lower numbers are considered immunogenic (lower than 66).

4—Number of HLA that presented percentile rank lower than 20 (high affinity).

5—Number of HLA that presented SMM IC50 lower than 500 nM (high affinity).

6—Number of HLA that presented NN IC50 lower than 500 nM (high affinity).

7—IFN-γ induction score. Positive values indicate IFN-γ induction.