Table 3.
Allergenicity, antigenicity and physicochemical parameters of the ZIKVac and with association with the proteinaceous adjuvants.
| Properties | Server | ZIKVac | ZIKVac + flagellin | ZIKVac + HBH | ZIKVac + RS09 | ZIKVac + 50S Rib |
|---|---|---|---|---|---|---|
| Length (aa) | ProtParam ExPASy Server | 564 | 847 | 771 | 579 | 702 |
| Probable non-allergen | AllergenFP Server | + | + | + | + | + |
| Probable antigen Virus, Thr: 0.4 | Vaxijen Server | + (0.4749) | + (0.5219) | + (0.4427) | + (0.4751) | + (0.4580) |
| Stable (Instability index) | ProtParam ExPASy Server | + (25.75) | + (26.81) | + (31.20) | + (26.27) | + (24.72) |
| Secondary structure | RaptorX Structure Prediction Server | 32%H, 26%E, 40%C | 39%H; 22%E; 37%C | 41%H; 18%E; 39%C | 31%H; 26%E; 41%C | 33%H; 24%E; 42%C |
| Solvent accessibility | RaptorX Structure Prediction Server | 41%E, 24%M, 34%B | 43%E; 25%M; 30%B | 45%E; 22%M; 32%B | 42% E; 23%M; 33%B | 43%E; 24%M; 32%B |
| Theoretical pI | ProtParam ExPASy Server | 9.82 | 9.4 | 9.65 | 9.77 | 9.29 |
| Molecular weight | ProtParam ExPASy Server | 58.78 kDa | 89.06 kDa | 81.12 kDa | 60.28 kDa | 73.02 kDa |
| Negatively charged residues | ProtParam ExPASy Server | 26 | 60 | 59 | 27 | 52 |
| Positively charged residues | ProtParam ExPASy Server | 51 | 77 | 87 | 51 | 67 |
| Extinction coefficients at 280 nm (if all pairs of Cys residues form cystines) | ProtParam ExPASy Server | 78,980 M−1 cm−1 | 80,470 M−1 cm−1 | 84,940 M−1 cm−1 | 80,470 M−1 cm−1 | 80,470 M−1 cm−1 |
| Extinction coefficients at 280 nm (if all pairs of Cys residues are reduced) | ProtParam ExPASy Server | 78,730 M−1 cm−1 | 80,220 M−1 cm−1 | 84,690 M−1 cm−1 | 80,220 M−1 cm−1 | 80,220 M−1 cm−1 |
| Estimated half life | ProtParam ExPASy Server |
30 h (mammalian reticulocytes, in vitro) > 20 h (yeast, in vivo) > 10 h (Escherichia coli, in vivo) |
30 h (mammalian reticulocytes, in vitro) > 20 h (yeast, in vivo) > 10 h (Escherichia coli, in vivo) |
30 h (mammalian reticulocytes, in vitro) > 20 h (yeast, in vivo) > 10 h (Escherichia coli, in vivo) |
30 h (mammalian reticulocytes, in vitro) > 20 h (yeast, in vivo) > 10 h (Escherichia coli, in vivo) |
30 h (mammalian reticulocytes, in vitro) > 20 h (yeast, in vivo) > 10 h (Escherichia coli, in vivo) |
| Aliphatic index | ProtParam ExPASy Server | 89.01 | 88.02 | 87.35 | 88.24 | 91.15 |
| GRAVY (Grand average of hydropathicity) | ProtParam ExPASy Server | 0.233 | 0.013 | 0.008 | 0.215 | 0.210 |